Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus.
Identifieur interne : 004737 ( Main/Exploration ); précédent : 004736; suivant : 004738Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus.
Auteurs : Khalid Amine Timani [République populaire de Chine] ; Qingjiao Liao ; Linbai Ye ; Yingchun Zeng ; Jing Liu ; Yi Zheng ; Li Ye ; Xiaojun Yang ; Kong Lingbao ; Jingrong Gao ; Ying ZhuSource :
- Virus research [ 0168-1702 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Cytométrie en flux, Délétion de gène, Humains, Lapins, Microscopie confocale, Noyau de la cellule (métabolisme), Nucléole (métabolisme), Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Signaux de localisation nucléaire, Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Protéines nucléocapside, Virus du SRAS.
- métabolisme : Noyau de la cellule, Nucléole, Protéines nucléocapside, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- Animaux, Cellules Vero, Cytométrie en flux, Délétion de gène, Humains, Lapins, Microscopie confocale, Protéines nucléocapside, Signaux de localisation nucléaire, Transfection.
English descriptors
- KwdEn :
- Animals, Cell Nucleolus (metabolism), Cell Nucleus (metabolism), Chlorocebus aethiops, Flow Cytometry, Gene Deletion, Humans, Microscopy, Confocal, Nuclear Localization Signals, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Rabbits, SARS Virus (genetics), SARS Virus (metabolism), SARS Virus (pathogenicity), Transfection, Vero Cells.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , metabolism : Nucleocapsid Proteins.
- chemical : Nuclear Localization Signals.
- genetics : SARS Virus.
- metabolism : Cell Nucleolus, Cell Nucleus, SARS Virus.
- pathogenicity : SARS Virus.
- Animals, Chlorocebus aethiops, Flow Cytometry, Gene Deletion, Humans, Microscopy, Confocal, Rabbits, Transfection, Vero Cells.
Abstract
A novel coronavirus (CoV) has recently been identified as the aetiological agent of severe acute respiratory syndrome (SARS). Nucleocapsid (N) proteins of the Coronaviridae family have no discernable homology, but they share a common nucleolar-cytoplasmic distribution pattern. There are three putative nuclear localization signal (NLS) motifs present in the N. To determine the role of these putative NLSs in the intracellular localization of the SARS-CoV N, we performed a confocal microscopy analysis using rabbit anti-N antisera. In this report, we show that the wild type N was distributed mainly in the cytoplasm. The N-terminal of the N, which contains the NLS1 (aa38-44), was localized to the nucleus. The C-terminus of the N, which contains both NLS2 (aa257-265) and NLS3 (aa369-390) was localized to the cytoplasm and the nucleolus. Results derived from analysis of various deletion mutations show that the region containing amino acids 226-289 is able to mediate nucleolar localization. The deletion of two hydrophobic regions that flanked the NLS3 recovered its activity and localized to the nucleus. Furthermore, deletion of leucine rich region (220-LALLLLDRLNRL) resulted in the accumulation of N to the cytoplasm and nucleolus, and when fusing this peptide to EGFP localization was cytoplasmic, suggesting that the N may act as a shuttle protein. Differences in nuclear/nucleolar localization properties of N from other members of coronavirus family suggest a unique function for N, which may play an important role in the pathogenesis of SARS.
DOI: 10.1016/j.virusres.2005.05.007
PubMed: 15992957
Affiliations:
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Le document en format XML
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<term>Flow Cytometry</term>
<term>Gene Deletion</term>
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<term>Nucleocapsid Proteins (metabolism)</term>
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<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Nucléole (métabolisme)</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Signaux de localisation nucléaire</term>
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<term>Virus du SRAS</term>
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<front><div type="abstract" xml:lang="en">A novel coronavirus (CoV) has recently been identified as the aetiological agent of severe acute respiratory syndrome (SARS). Nucleocapsid (N) proteins of the Coronaviridae family have no discernable homology, but they share a common nucleolar-cytoplasmic distribution pattern. There are three putative nuclear localization signal (NLS) motifs present in the N. To determine the role of these putative NLSs in the intracellular localization of the SARS-CoV N, we performed a confocal microscopy analysis using rabbit anti-N antisera. In this report, we show that the wild type N was distributed mainly in the cytoplasm. The N-terminal of the N, which contains the NLS1 (aa38-44), was localized to the nucleus. The C-terminus of the N, which contains both NLS2 (aa257-265) and NLS3 (aa369-390) was localized to the cytoplasm and the nucleolus. Results derived from analysis of various deletion mutations show that the region containing amino acids 226-289 is able to mediate nucleolar localization. The deletion of two hydrophobic regions that flanked the NLS3 recovered its activity and localized to the nucleus. Furthermore, deletion of leucine rich region (220-LALLLLDRLNRL) resulted in the accumulation of N to the cytoplasm and nucleolus, and when fusing this peptide to EGFP localization was cytoplasmic, suggesting that the N may act as a shuttle protein. Differences in nuclear/nucleolar localization properties of N from other members of coronavirus family suggest a unique function for N, which may play an important role in the pathogenesis of SARS.</div>
</front>
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<name sortKey="Zeng, Yingchun" sort="Zeng, Yingchun" uniqKey="Zeng Y" first="Yingchun" last="Zeng">Yingchun Zeng</name>
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<name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name>
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<country name="République populaire de Chine"><region name="Hubei"><name sortKey="Timani, Khalid Amine" sort="Timani, Khalid Amine" uniqKey="Timani K" first="Khalid Amine" last="Timani">Khalid Amine Timani</name>
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